Platelet Rich Plasma-platelet Concentrate (PRP-PC) Analysis

blood platelet Rich Plasma-platelet Concentrate (PRP-PC) AnalysisQuality assessment of platelet concentrates nimble at Dr. Pinnameneni Institute of Medical Sciences and investigate Foundation Dr. Anusha A.MBackgroundPlatelet liberal plasma-platelet concentrate (PRP-PC) were prepared and their whole feel variables were evaluated.Material and methodsIn this study platelet products were prepared using platelet rich plasma method. After preparation the products were transferred to platelet incubator and agitated. Their quality was assessed after 24 hours of preparation using the following parameters volume of the platelet concentrate, platelet debate, erythrocyte contaminant, morphology and pH.ResultsVolume 90% of the RDP was slowness between 50 to 70 ml, 4% below 50 and 6% above 70 ml. The run jibe well in both the methods and 85% of RDP had a count of above 5.5 x 1010, 15% had below 5 x 1010. Ph 56% of the RDP had of 6.3 to 6.5, 33% had 6.6 to 7.0 and 6% at 6.2 and 5% above 7.0. Appearance 86% was light straw colored, 3% light pint, 6% pink and 5% red.ConclusionDuring the transshipment center of platelet concentrates there is progressive loss in capacity of survival and function of platelets. In order to maximize the preservation of platelet viability it is best to waive PRP to repose at agency temperature for 1-2 hours and then transfuse as soon as possible. To maximize the sanative values of platelet concentrates quality control is indispensable and helps to identify trouble shooting in procedures. In resultant more than 95% of the RDPs prepared meet the standard.KeywordsPlatelet rich plasma-platelet concentrate, quality parameters, platelet countIntroduction transfusion medicine has over the years evolved to assume a complex medical discipline that aided or modified patient care. line of products donation culture has not been fully imbibed in our society and homologous lineage is usually in short supply in the blood banks with its hearer c onsequences in patient management1.Platelet transfusion therapy has played an important role in the management of patients 2,3. Today, platelet concentrates are prepared from whole blood each by assortedial centrifugation buffy coat-derived platelet concentrates (BC) or by platelet rich plasma- platelet concentrates (PRP-PC) and plateletpheresis (4,5).There are several methods for quality control of platelet components including electric cell counting, pH, volume and morphology.6.7.8.The aim of this study was to evaluate the quality of platelets during the storage of platelet concentrates derived from PRP-PCs and whether patients got adequate therapeutically useful amount of platelets.Materials and MethodsThe present study was conducted at blood bank, PSIMS RF, Andhra Pradesh, India. The study was carried out on 100 patients. Platelet products were prepared from whole blood using platelet rich plasma method. After preparation these were stored in platelet incubator and agitated. Their quality was assessed after 24 hours of preparation using the following parameters volume of the platelet concentrate, platelet count, erythrocyte contamination, morphology and pH. For the study, samples were taken from the element of tubing in the platelet concentrate bag to maintain sterility inside the bag.VolumeThe volume of the platelet concentrates were measured by deducting the volume of the abandon bag from the volume of the platelets concentrate bag in ml. The measurements were recorded.pHp H of the platelet concentrate units were check out by the use of semi-quantitative dipsticks ( Bayers multistix strips)Total Platelet CountPlatelet count was done by 2 methodsAutomated method by using fully automated analyzer Sysmex KX-21 to assess the quality o the platelets. Counting was based on impedance technology.Manual method using counting chamber.RBC contaminationPlatelet concentrate unit was checked by visual inspection or various colours.MorphologyMorphology was analyz ed by staining smear by leishman stain.Results1. Volume95% of PRP-PC was weighing between 55 to 75 ml and 5% below 55.pH76% of the PRP-PC had of 6.3 to 6.5, 20% had 6.6 to 7.0 and 4% below 6.3.Total Platelet CountThe count correlated well in both the methods and 90% of PRP-PC had a count of above 5.0 x 1010 and 10% below 5 x 1010.4. RBC contamination92% was light straw colored, 4% light pint and 4% pink.Morphology94% of the platelets were discoid, 4% spherical and 1% fragmented.DiscussionThe potential of transfused platelets to hand out and function is dependent on ex-vivo and in-vivo factors. The percentage of platelets that maintain discoid form is a primary and simple indicator for the quality of the stored platelet concentrates. PCs been gently prepared and then immediately transfused without a storage interval have high retrieval, good survival and conserved function.Quality assessment of platelet concentrates is an important step to evaluateex-vivofunctional viability of plat elet concentrates and post transfusion recovery and survival in donee. Various variables are used for routinequality assessment of platelet concentrates such as volume, platelet count, morphology, RBC contamination and pH.ConclusionDuring storage, platelet concentrates gradually lose the capacity to survive and function. In order to preserve platelet viability, PRP should be allowed to rest at room temperature, for 1-2 hours and transfused as soon as possible thereafter. There is a need to improve the quality of the platelet concentrates being prepared to get maximum therapeutic values. Doing quality control is essential and it is not only valuable in itself but also helps in identify trouble shooting of the procedures. In conclusion more than 95% of the PRP-PC prepared met the standards.ReferencesOlaitan PB, Onah I I, Ogbonnaya I S. Preliminary reports of autologous blood transfusion in a plastic surgery unit. Tropical Doctor.2006 36 20-21Snyder EL, Hezzey A, Katz AJ, Bock J (1981) Occurrence of the release reaction during preparation and storage of platelet concentrates. Vox Sang 41172-177.Heaton WA, Rebulla P, Pappalettera M, Dzik WH (1997) A comparative analysis of different methods for routine blood component preparation. Transfus MedRev 11116-129.Fijnheer R, Pietersz RN, de Korte D, Gouwerok CW, Dekker WJ, et al. (1990)Platelet activation during preparation of Platelet Concentrate A comparison of Platelet Rich Plasma and the buffy coat methods. Transfusion 30 634-638.Jerad S, Prane K (1997) The Platelet Storage lesions. Transfusion Medicine Reviews 2 130-144.Dijkstra-Tiekstra MJ, Pietersz RN, Huijgens PC (2004) Correlation Between the extent of platelet activation in platelet concentrates and in vitro and in vivo parameters. Vox Sang 87 257-263.Kamath S, Blann AD, Lip GY (2001) Platelet activation assessment andquantification. Eur Heart J 22 15611571.Albanyan AM, Murphy MF, Rasmussen JT, Heegaard CW, Harrison P (2009)Measurement of phosphatidylserine pi c during storage of platelet concentrates using the novel probe lactadherin a comparison study with annexin V. Transfusion 49 99-107.Rinder HM, Smith BR. In vitro evaluation of stored platelets Is there try for for predicting post-transfusion platelet survival and function?Transfusion.20034326Holme S. Storage and quality assessment of platelets.Vox Sang.19987420716.